Poster Titles Please feel free to download the available posters
- Protein N- and C-terminal Sequencing Using Electron Transfer Dissociation Mass Spectrometry. Authors:Hao, Zhiqi and Hühmer, Andreas.
- Intact protein N-terminal Sequencing by Mass Spectrometry; Alternatives to Edman Degradation Authors:Mohtashemi, I., Hao, Z. and Hühmer, A.
Thank you to everyone who attended our Technical Workshop!
Sunday, February 8th
7:40 PM - 8:40 PM
Room 202
Memphis Cook Convention Center
Topic 1. Quantification of Phosphoproteins:
New experimental approaches for investigating cellular proliferation
To enhance understanding of complex regulation of phosphorylation in cellular proliferation, we describe a new, integrated workflow for the simultaneous identification and quantification of phosphoproteins and phosphopeptides. This workflow features a multiplexed quantification strategy that uses isobaric mass labels and high-resolution, high-mass-accuracy LC-MSn to resolve and quantify protein expression and phosphoprotein changes in cell lines. This workflow provides resolution, throughput, and accuracy for quantification of phosphoprotein variants that is superior to traditional methods.
Topic 2. Sample preparation strategies
for detection of proteotypic peptides by ETD
The introduction of electron transfer dissociation (ETD) for peptide sequencing applications has sparked renewed interest in using alternative proteolytic enzymes to complement the commonly used trypsin in proteomics applications. We combine a pressure cycling technology, the cleavage specificity of various alternative enzymes, and optimal detection parameters on a Fisher Scientific LTQ XL ETD mass spectrometer to increase the confidence for peptide identifications and the number of available proteotypic peptides in a given sample. We discuss the proposed alternative sample preparation workflows in the context of various Proteomics applications.
Come enjoy refreshments while you learn about these workflows and more of Thermo Scientific integrated solutions for proteomics and quantitative biology.
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