Engineered for easy setup and maximum productivity, the PepFinder
Kit enables rapid implementation of automated nanospray LC/MSn methods for
Finnigan LCQ and LTQ systems. The PepFinder Kit boosts sensitivity by
concentrating peptides. The PepFinder Kit is a convenient time-saving,
results-enhancing tool for protein analysis.
The PepFinder Kit for fast, sensitive, robust nanospray LC/MS applications, enabling rapid implementation of automated nanospray LC/MSn methods for Finnigan LCQ and LTQ systems. Engineered for easy setup and maximum productivity, all PepFinder Kit components come pre-assembled, minimizing setup time and optimizing the system flowrate with minimal effort. Materials, plumbing configurations, and connection of flowpath components are all engineered for optimum out-of-the-box performance.
Nanospray LC/MS is the definitive method for protein identification by mass spectrometry. An unknown sample, typically from a 2D gel, is proteolytically digested and loaded on a nanobore separation column at very low flowrate. Peptides are resolved and sequenced, yielding a protein identification. Nanobore columns, typically 75μm internal diameter, require very low flowrates and are prone to plugging and fouling.
PepFinder Kit eliminates all of these issues. Incorporation of a peptide trap allows sample loading and desalting at high flowrates, greatly reducing overall analysis times. It boosts sensitivity by concentrating peptides from dilute sample solutions. Further, the peptide trap dramatically improves overall method robustness, protecting the fragile nanobore column.
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