The analysis of complex protein mixtures is one of the most challenging tasks in the area of proteomics. The key requirements of mass spectrometry based methods are a wide dynamic range, outstanding mass accuracy, fast cycle times for MS/MS experiments, and high sensitivity. The LTQ Orbitrap XL™ meets all of these requirements and provides the most confident protein identification.

The LTQ Orbitrap XL Provides:

• Superior mass accuracy for lower false positive rates (FPRs)
• High sensitivity and dynamic range leading to more protein identifications
• Parallel acquisition mode for optimized productivity
• Excellent MS/MS sensitivity

Base peak chromatogram of E. coli total enzymatic digest

Experimental Method

• E. coli total digest (500 ng)
• Online nano LC-MS and MS/MS
• Optimized productivity with parallel detection
• Orbitrap mass analyzer full scan with Ion Trap Top 7 Data Dependent™ MS/MS
• Dynamic exclusion

Orbitrap mass analyzer full scan MS at RT 89.4 min with zoom-in.