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Doping Analysis: From Screening to Final Confirmation
Doping Analysis

ITQ 900 GC/MS
ITQ 900 with TRACE GC Ultra

TSQ Quantum Ultra with Accela
TSQ Quantum Ultra with Accela

DSQ II
DSQ II with TRACE GC Ultra

LTQ Orbitrap
LTQ Orbitrap

DELTA V with TRACE GC Ultra
DELTA V Isotope Ratio MS with TRACE GC Ultra, GC-C/TC III
Combustion and Autosampler AS3000

DFS with TRACE GC Ultra
DFS High Resolution GC/MS with Dual TRACE GC Ultra
and TriPlus Autosampler XT
Screening and Confirmation for
Performance Enhancing Substances

• Anabolic Steroids
• Peptides Hormones
• Narcotic Analgesics, Beta-Blockers
• Diuretics
• Stimulants

Figure 1
Comparison of urinary T/E ratios and δ13C testosterone values determined by GC/Isotope ratio MS after ingestion of 40 mg testosterone-undeconate. Values above this range give direct evidence for synthetic testosterone excreted in urine (dotted line)
Courtesy of German Sport University Cologne – Center for Preventive Doping Research / Institute of Biochemistry.

Analysis and confirmation of synthetic testosterone
The administration of synthetic testosterone is detected by the testosterone/epitestosterone (T/E) ratio in urine using GC/MS. Confirmation is performed by GC/Isotope Ratio MS. While the surplus of synthetic testosterone is excreted rapidly in the urine, as evidenced by the fast return of the T/E ratio to a value below 3 (red curve), the carbon atom 13C/12C ratio of testosterone (δ13C) stays at the value of synthetic testosterone for a much longer period of time (blue bars) due to supressed production of testosterone. The 13C/12C ratio of synthetic testosterone differs from the 13C/12C ratio of endogenous testosterone. The decrease back to the normal 13C/12C ratio of testosterone starts with the recovered production of endogenous testosterone and is delayed by almost 12 hours.